1103-Synthetic Rewriting of the IGH Locus
Researchers have successfully developed SynIgh, a synthetic human immunoglobulin heavy chain locus designed de novo to explore and expand antibody diversity. This modular construct was engineered using genes from global human populations and integrated into a mouse model to demonstrate functional V(D)J recombination. To overcome the structural instability of highly repetitive sequences, the team utilized a specialized DNA stabilizer during the assembly process in microbial hosts. A key feature of the project is the mSCRaMbLE system, which allows for programmable, inducible shuffling of the…
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