216-All-Optical In Situ Histology of Heterogeneous Tissue

Paper Talk · Nov 8, 2025 · 25m

The article details the development and application of a robotic nonlinear optical system designed for all-optical in situ histology of heterogeneous tissues. This innovative system combines iterative multiphoton microscopy with spatiotemporal line-focused laser micromachining to achieve submicrometer resolution 3D imaging and reconstruction of large-scale samples, such as the intact murine craniocerebral system. A key advancement is the use of spatiotemporal focusing to increase machining efficiency by 100-fold and the implementation of a smart, autonomous control pipeline for multi-day…