1367-Whole-Transcriptome Isoform-Resolved Spatial Imaging
The paper introduce RT&T-AMP-MERFISH, a cutting-edge spatial transcriptomics technique that enables isoform-resolved imaging across the entire transcriptome. By combining in situ RNA amplification with multiplexed error-robust fluorescence in situ hybridization, researchers can now detect approximately 33,000 distinct RNAs at single-cell and subcellular resolution. This methodology was successfully applied to mouse brain tissues, creating a high-resolution atlas that identifies complex gene programs and cell-cell communication networks. The data specifically highlight how transcript isoform…
The skinny
The skinny isn't ready yet — notes appear once the transcript is processed.