316-TIRTL-seq: Deep Paired TCR Repertoire Sequencing
The paper introduces TIRTL-seq (throughput-intensive rapid TCR library sequencing), a novel experimental and computational methodology designed to address the trade-offs between affordability and detailed data collection in T cell receptor sequencing. This new approach combines the high throughput and quantitative precision of older bulk sequencing methods with the critical capacity for acquiring paired TCR alpha and beta chain sequences, a feature traditionally limited to expensive single-cell technologies. TIRTL-seq is highly affordable and scalable, operating in a 384-well plate format to…
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