264-snATAC-seq in the Adult Mouse Brain

Paper Talk · Nov 25, 2025 · 15m

The paper details a comprehensive study that generated a candidate cis-regulatory DNA element (cCRE) atlas of the adult mouse brain through single-cell analysis of chromatin accessibility in over 2.3 million cells. Researchers used single-nucleus assay for transposase-accessible chromatin followed by sequencing (snATAC-seq) across 117 anatomical dissections to identify approximately 1 million cCREs and delineate 1,482 distinct brain cell populations. The work integrated this data with single-cell RNA sequencing to infer gene regulatory networks (GRNs) in over 260 subclasses of brain cells…