223-PrecisION: Native Top-Down Mass Spectrometry Software

Paper Talk · Nov 11, 2025 · 20m

The article details the development and application of precisION, an interactive, open-source software package for analyzing data from native top-down mass spectrometry (nTDMS). This new computational framework uses a data-driven fragment-level open search to overcome the limitations of current nTDMS methods, which often fail to detect uncharacterized or low-abundance protein modifications (PTMs) within intact protein complexes. The authors successfully applied precisION to therapeutically relevant targets—including PDE6, ACE2, osteopontin (SPP1), and a GABA transporter (GAT1)—to discover…