293-3D Deep-STARmap and Deep-RIBOmap in Thick Tissues
The paper introduces novel imaging platforms, Deep-STARmap and Deep-RIBOmap, designed for scalable, single-cell spatial transcriptomics and translatomics within thick, three-dimensional (3D) tissue blocks, overcoming limitations of traditional thin-sectioning methods. The researchers detail the technological advancements, including scalable probe synthesis and robust complementary DNA crosslinking, which enable the quantification of thousands of gene transcripts and their corresponding translation activities. The utility of these methods is demonstrated through comprehensive studies in mouse…
The skinny
The skinny isn't ready yet — notes appear once the transcript is processed.