120-Stereo-seq V2: Spatial mapping with high resolution
This article introduces Stereo-seq V2, an advanced spatial transcriptomics method designed for high-resolution total RNA mapping on formalin-fixed, paraffin-embedded (FFPE) tissue sections, which are commonly used in clinical settings. This updated technique utilizes random priming to overcome the limitations of traditional methods on degraded FFPE RNA, enabling the capture of non-coding RNAs (ncRNAs) and providing uniform gene body coverage. The source highlights the method's capability to profile host and pathogen transcriptomes simultaneously, as demonstrated in a Mycobacterium…
The skinny
The skinny isn't ready yet — notes appear once the transcript is processed.