1194-Perturb-DBiT: CRISPR Screening in Native Tissue
The paper describe Perturb-DBiT, a novel biotechnology designed for spatially resolved CRISPR screening within intact tissue environments. By utilizing microfluidic barcoding, the method simultaneously sequences single guide RNAs (sgRNAs) and the total transcriptome, including both coding and noncoding RNAs. This approach allows researchers to link specific genetic edits to changes in cellular phenotypes and gene regulation without losing the spatial context of the tissue. The study demonstrates the platform's effectiveness in tumor models, successfully identifying genetic drivers of…
The skinny
The skinny isn't ready yet — notes appear once the transcript is processed.