1673-WHOLISTIC Imaging of Body-Wide Cellular Circuits

Paper Talk · Oct 14, 2026 · 24m

The paper introduces WHOLISTIC, a novel whole-organism live-imaging framework designed to simultaneously record second-timescale cellular activity across all tissues in transparent vertebrates like larval zebrafish. By integrating volumetric fluorescence microscopy, pancellular calcium indicators, and advanced motion-correction algorithms, this system overcomes previous optical barriers to map dynamic intercellular interactions body-wide. The platform successfully identifies functional tissue ensembles, uncovers novel muscle synergies and nerve innervations, and tracks inter-organ coupling…