746-INSTALL: Immune Evasive Kilobase Scale Genome Writing
This paper introduces INSTALL, a novel genome-engineering method that utilizes circular single-stranded DNA (cssDNA) to achieve large-scale genetic integration. Traditional techniques rely on double-stranded DNA, which often triggers toxic innate immune responses and limits the efficiency of non-viral gene delivery. By pairing these "immune-stealth" DNA donors with recombinase enzymes, the researchers successfully inserted kilobase-scale sequences into human cells and mice with high fidelity. The study demonstrates that INSTALL bypasses cellular defense mechanisms like the cGAS-STING pathway…
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