256-Benchmarking Spatial Transcriptomics Platforms

Paper Talk · Nov 22, 2025 · 13m

This article provides a systematic evaluation of four advanced, commercially available high-throughput spatial transcriptomics (ST) platforms with subcellular resolution: Stereo-seq v1.3, Visium HD FFPE, CosMx 6K, and Xenium 5K. To establish a robust benchmark, the researchers generated serial tissue sections from human cancer samples (colon adenocarcinoma, hepatocellular carcinoma, and ovarian cancer) and used CODEX protein profiling and single-cell RNA sequencing (scRNA-seq) as orthogonal ground truth datasets. The study comprehensively assesses platform performance across technical metrics…