348-mTORC2 Recognizes AGC Kinases by Long-Range Docking

Paper Talk · Dec 30, 2025 · 14m

This article focuses on mTORC2, a protein kinase complex critical for diverse cellular functions, including growth, metabolism, and proliferation, whose dysregulation is implicated in various diseases like cancer. The authors aimed to determine the structural basis for how mTORC2 selectively recognizes and phosphorylates its substrates, particularly Akt, a crucial signaling protein. Using a combination of cryo-electron microscopy (cryo-EM), chemical semisynthesis, and cross-linking mass spectrometry, the study details two key structural interfaces between Akt and the mTORC2 subunit mSin1…