124-PathoPlex: Spatial Proteomics Mapping
This paper details the introduction and validation of PathoPlex, a scalable and open-source framework for highly multiplexed imaging and analysis of formalin-fixed paraffin-embedded (FFPE) tissue specimens. This technology significantly advances spatial biology by enabling the detection of over 120 protein markers at subcellular resolution, exceeding the capabilities of existing commercial systems. PathoPlex combines iterative indirect immunofluorescence with a custom-built, low-cost 3D printing-based automation system for high-throughput sample processing and utilizes the accompanying…
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