133-Highly Multiplexed 3D Tumor Profiling

Paper Talk · Oct 12, 2025 · 30m

This article introduces a novel technique called three-dimensional cyclic immunofluorescence (3D CyCIF) for high-plex, high-resolution imaging of cell states and immune niches within human tumor tissues. The authors demonstrate that traditional thin tissue sections, typically 5 µm thick, result in fragmented cells and inaccurate phenotyping, whereas sections eightfold to tenfold thicker (30–40 µm) enable accurate morphological assessment of intact cells, organelles, and cell-to-cell contacts. Using 3D CyCIF on melanoma samples, the study reveals detailed tumor microarchitecture, including…