384-FX-Cell: ScRNA Sequencing for Challenging Plant Samples
Researchers have developed a suite of new protocols, collectively called FX-Cell, to improve single-cell RNA sequencing in diverse plant species. These methods utilize coagulant fixatives to stabilize cells, allowing them to withstand the high temperatures and mechanical forces needed to break down rigid cell walls. A critical component of the workflow involves a column-based purification step that removes harmful RNases from digestive enzymes, thereby preserving the integrity of the genetic data. This approach enables the study of cryopreserved field samples and reveals accurate wounding…
The skinny
The skinny isn't ready yet — notes appear once the transcript is processed.