003-Perturb-Multimodal Imaging and Sequencing
This resource article introduces Perturb-Multimodal (Perturb-Multi), an innovative platform integrating imaging and sequencing for pooled genetic screening in intact mammalian tissues. The method captures in situ genotypes and multiple RNA-protein phenotypes through RCA-MERFISH, and enables in vivo CRISPR screening while preserving complete transcriptomes through fixed-cell Perturb-seq. The article elaborates on these technical innovations, using mouse liver as an example to demonstrate applications in exploring genetic regulation of liver function, cellular zonation, stress responses, and…
The skinny
The skinny isn't ready yet — notes appear once the transcript is processed.