364-RADIANT: Highly Multiplexed Volumetric Mapping
This research introduces RADIANT, a groundbreaking optical imaging method designed to map numerous proteins simultaneously within large, three-dimensional biological volumes. Conventional fluorescence microscopy is hindered by a "color barrier" that limits simultaneous imaging to a few targets, while existing high-multiplex methods are typically restricted to thin tissue sections. To overcome these obstacles, the authors utilize stimulated Raman scattering (SRS) microscopy combined with specially engineered MARS dyes, which feature narrow vibrational peaks that allow for the clear separation…
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