160-Spatial Atlas of Developing Human Intestinal Fibroblasts

Paper Talk · Oct 23, 2025 · 15m

This paper utilizes single-cell RNA-sequencing (scRNA-seq) and Xenium spatial transcriptomics to create a detailed cellular and positional atlas of the developing human intestine, specifically focusing on previously undefined fibroblast populations (mesenchyme/stroma). The authors identify and map four distinct fibroblast subtypes: subepithelial cells (SECs), lamina propria fibroblasts (LPFs), submucosal fibroblasts (SMFs), and rare CXCL13+ fibroblasts—across various developmental stages and into adulthood. Furthermore, this atlas is employed to benchmark human intestinal organoids (HIOs)…