1659-Genome-Scale Perturb-Seq Mapping of Human T Cell

Paper Talk · Oct 9, 2026 · 25m

Researchers developed a massive genome-scale perturb-seq platform to map the regulatory networks of 22 million primary human CD4+ T cells. By systematically knocking down over 12,000 genes across different activation states, they created a dynamic atlas that identifies how specific regulators control immune programs and cytokine production. The study demonstrates that gene regulatory effects change significantly depending on whether a cell is at rest or stimulated. Furthermore, the authors successfully used this data to predict the regulators responsible for T cell polarization and…