095-OpenCell: Mapping Human Cell Organization
This research introduces OpenCell, a comprehensive resource developed through high-throughput CRISPR-mediated genome editing to map the architecture of the human proteome. The project generated 1310 fluorescently tagged human cell lines, enabling live-cell imaging of protein localization and immunoprecipitation-mass spectrometry (IP-MS) to characterize protein interactions. By combining these advanced experimental techniques with unsupervised machine learning for image analysis, the researchers created a vast dataset that offers insights into individual protein functions and the general…
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