1125-Multi-Modal Profiling of Antigen-Specific CD4+ T Cells

Paper Talk · Jun 13, 2026 · 24m

This research introduces APMAT-CD4, a high-throughput platform designed to identify and characterize antigen-specific CD4+ T cells at single-cell resolution. The authors developed a modular system using class II single-chain trimer libraries to overcome the technical challenges of profiling helper T cells across multiple dimensions, including TCR sequences, transcriptomes, and MHC restriction. The technology was validated by screening SARS-CoV-2 spike proteins in a longitudinal cohort, revealing how immunogenicity scores relate to antibody production and disease progression. Furthermore, the…