654-mFISH3D: AI-Driven Whole-Organ Spatial Mapping

Paper Talk · Mar 17, 2026 · 25m

Researchers have developed mFISH3D, a sophisticated imaging technique that allows for the simultaneous visualization of multiple mRNA targets within intact, large-scale biological tissues. By combining fluorescence in situ hybridization with an AI-driven analysis platform called ZenCell, the workflow achieves precise single-cell mapping across entire organs like the mouse brain. This methodology overcomes historical limitations in depth and scalability, proving effective in diverse specimens including mouse embryos, kidneys, and even archived human brain tissue. The integration of…