1380-SPACE-seq: Spatial CRISPR Lineage Tracing
Researchers have developed SPACE-seq, a versatile technology that integrates CRISPR-based barcoding with spatial transcriptomics to simultaneously map a cell’s ancestry, state, and location. By applying this method to liver cancer models, the authors discovered that clonally related tumor cells diversify into multiple distinct phenotypic states. The study also identifies a bidirectional crosstalk between tumor cells and the surrounding stroma, specifically showing how the protein SELENOP acts as a brake on fiber-producing cell expansion. Beyond cancer, SPACE-seq was used to reconstruct 3D…
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