276-Compressed Perturb-seq for Genetic Screens

Paper Talk · Nov 29, 2025 · 19m

The article introduces a novel, cost-effective method called "compressed Perturb-seq" for large-scale genetic screening of regulatory circuits. This technique modifies the conventional Perturb-seq method by employing algorithmic compressed sensing principles, allowing for measurement of random combinations of genetic perturbations (either through "cell-pooling" or "guide-pooling") and subsequent computational decompression using a new inference method, FR-Perturb. The authors demonstrate that compressed Perturb-seq achieves accuracy comparable to conventional methods while offering…