190-Spatial Proteomics Reveals Primary Cilia Heterogeneity

Paper Talk · Oct 29, 2025 · 14m

This article utilizes antibody-based spatial proteomics and 3D confocal imaging to map the locations of 715 proteins within human primary cilia across three different cell lines, creating a spatial cilia atlas. Key findings highlight the extensive heterogeneity of the ciliary proteome, noting that 69% of identified proteins are cell-type specific and 78% show variation across individual cilia, suggesting that cilia function as adaptable sensors. The research identifies 91 novel ciliary proteins, proposes CREB3 as a ciliopathy candidate gene associated with a complex syndromic presentation…