296-PRISM: Single-Round High-Multiplex Spatial RNA Imaging
The paper introduces PRISM (profiling of RNA in situ through single-round imaging), a new high-multiplex fluorescent in situ hybridization (FISH) method designed to make spatial RNA imaging more accessible and efficient by overcoming the limitations of conventional microscopy. PRISM expands coding capacity by using color-intensity barcoding within a single round of staining, allowing for up to 64-plex detection without complex, expensive fluidics-dependent instrumentation. The article demonstrates PRISM’s robustness by applying it to create detailed spatial and quasi-3D atlases of diverse…
The skinny
The skinny isn't ready yet — notes appear once the transcript is processed.