766-Functional Mapping Mammalian Whole Brain via Perturb-seq

Paper Talk · Apr 9, 2026 · 22m

This study introduces a comprehensive functional atlas of the mammalian brain by utilizing an enhanced in vivo Perturb-seq platform to map the effects of 1,947 disease-associated genes. By profiling over 7.7 million individual cells across the mouse brain, researchers identified how genetic loss-of-function impacts specific neuronal populations and transcriptional programs. The findings highlight that genetic essentiality is highly context-dependent, with certain mutations causing cell-type-specific depletion while others drive broad responses. Notably, the research reveals that closely…