Latest / Beyond Labels Premium / Episode #45: Can the "Real Life Jurassic Park Scientist" Save Us from Glyphosate & Disease?

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Episode #45: Can the "Real Life Jurassic Park Scientist" Save Us from Glyphosate & Disease? · Beyond Labels Premium

• 0:00
  SPEAKER_01: Given sufficient time, and I'll say sufficient time, I'm not measuring in terms of years, but in terms of months, you can remove most of the detectable glyphosate with exposure to sufficient concentrations of this consortium of paleo restore.
• 0:19
  SPEAKER_01: In our study, uh, we predicted that over the six-month period, most of the measurable glyphosate would that would not be present.
• 0:27
  SPEAKER_01: It will be degraded by the microbial consortium.
• 0:32
  SPEAKER_00: Hello, everybody.
• 0:33
  SPEAKER_00: Welcome to another episode of Beyond Labels.
• 0:35
  SPEAKER_00: I'm Dr.
• 0:35
  SPEAKER_00: Cena McCullough, and I'm here today with Joel Salatin.
• 0:39
  SPEAKER_00: And we have a very special guest.
• 0:42
  SPEAKER_00: We're joined by Dr.
• 0:43
  SPEAKER_00: Raul Cano.
• 0:44
  SPEAKER_00: And some of you may have heard his name before.
• 0:48
  SPEAKER_00: He was dubbed the real world Jurassic Park scientist.
• 0:53
  SPEAKER_00: Today he's going to be talking to us about how he has been working to create a probiotic using ancient microbes in order to regenerate the soil and which would ultimately regenerate the human microbiome.
• 1:09
  SPEAKER_00: Now, one thing that I think is really cool about Dr.
• 1:11
  SPEAKER_00: Hanno is that he was figuring out how to restore the microbiome before the term microbiome was even coined, right?
• 1:21
  SPEAKER_00: So he was so far ahead of the game.
• 1:23
  SPEAKER_00: I think one of the reasons why I think the work he does is so critical is because now we know that human disease is largely associated with loss of microbiome, a loss of the numbers of microbes, and also loss of diversity in the gastrointestinal tract, for instance.
• 1:43
  SPEAKER_00: So what he's doing, the work he's doing to create, to basically restore the microbiome, is actually gonna help us be healthier not only for the soil, like in an environment, but also as humans.
• 1:59
  SPEAKER_00: He's also gonna talk today about how he has discovered a way to degrade glyphosate all the way down into CO2, water, and phosphate.
• 2:12
  SPEAKER_00: So he's actually able to, for the first time ever, the first person to completely degrade glyphosate.
• 2:19
  SPEAKER_00: Now, as you can imagine, Dr.
• 2:20
  SPEAKER_00: Candle has a very long resume and list of accomplishments.
• 2:24
  SPEAKER_00: They're all included in the description of this talk.
• 2:28
  SPEAKER_00: But I've just real quickly, I wanted you to know that he is the president and CEO of Paleobiotica, which is a company that focuses on agricultural and environmental solutions.
• 2:38
  SPEAKER_00: He has a PhD in microbiology from the University of Montana, and he holds two master's degrees, one in fungal genetics and the other in clinical microbiology.
• 2:48
  SPEAKER_00: He has earned certifications in public health and environmental microbiology as well.
• 2:53
  SPEAKER_00: Okay, I'm gonna make a real world happen here.
• 2:55
  SPEAKER_00: I had to rescue my daughter from the dog's barking and look her up from her nap.
• 2:59
  SPEAKER_00: So we're back with Dr.
• 3:00
  SPEAKER_00: Cano and Jill Salatin.
• 3:03
  SPEAKER_00: So as I was saying, uh, Dr.
• 3:05
  SPEAKER_00: Cano, together with Robin Steele, created a company that focuses on isolating and characterizing these ancient microorganisms from amber, deep sea cores, and other ancient environments.
• 3:17
  SPEAKER_00: He's actually resuscitated dormant microbes and created a library of over 2,000 different types of bacteria and fungi.
• 3:25
  SPEAKER_00: Um, he basically applies this microbiome restoration solutions to, like we said, to restore the soil.
• 3:33
  SPEAKER_00: And to identify likely drilling sites for petroleum and natural gas exploration.
• 3:39
  SPEAKER_00: Um his bio goes on and on.
• 3:41
  SPEAKER_00: It's it's amazing.
• 3:42
  SPEAKER_00: You should check it out.
• 3:43
  SPEAKER_00: But I would love to just go ahead and have you hear from him.
• 3:46
  SPEAKER_00: Welcome to the show, Dr.
• 3:48
  SPEAKER_00: Cano.
• 3:50
  SPEAKER_01: Thank you very much, Zina.
• 3:52
  SPEAKER_01: Thank you.
• 3:52
  SPEAKER_01: Very an honor to be in your show.
• 3:55
  SPEAKER_01: Thank you.
• 3:58
  SPEAKER_00: So, Joel, do you want to start out with the first question?
• 4:02
  SPEAKER_02: Well, um, after that uh distinguished resume, uh you know, my my head's just buzzing with with things.
• 4:11
  SPEAKER_02: Uh, you know, I've I've been um you know very friendly with seed savers.
• 4:17
  SPEAKER_02: Um, you know, at our home, we have a a tomato.
• 4:21
  SPEAKER_02: We don't know what the name of it was, but it was it was Tree, my wife's uh great great grandmother's, and it's been passed down now, I think six generations, this this tomato seed.
• 4:34
  SPEAKER_02: And we still we still grow them every year.
• 4:36
  SPEAKER_02: We keep the seeds, we save the seeds.
• 4:37
  SPEAKER_02: So so uh Dr.
• 4:39
  SPEAKER_02: Um, uh you know, my my first question is how do these microbes survive?
• 4:47
  SPEAKER_02: Where I mean you talk about drilling and finding them in ancient places.
• 4:52
  SPEAKER_02: Do you how do you breathe life back into them?
• 4:58
  SPEAKER_01: Well, most you know, microorganisms in general have a remarkable repertoire of survival strategies.
• 5:08
  SPEAKER_01: Some of them become so water-free that essentially it inhibits any kind of metabolic processes, most of which take place in the presence of water.
• 5:21
  SPEAKER_01: So there's really no hydrolysis.
• 5:23
  SPEAKER_01: Some of them have some what they call themselves or resuscitation genes, which allow them to spend thousands of years in dormancy, and then uh once the proper environmental conditions are set or are available, the resuscitation genes will kick in and restore uh viability.
• 5:47
  SPEAKER_01: Uh, you know, there are a lot of organisms, you know, even in clinical microbiology, which are called viable but not culturable.
• 5:55
  SPEAKER_01: That means that they're alive, but they cannot be cultured using our techniques that we know of.
• 6:00
  SPEAKER_01: So uh, and many organisms can be found in in environments, for example, amber, that are highly dehydrating.
• 6:12
  SPEAKER_01: So they essentially suck up all the water from the cell, and then basically they are in suspended animation.
• 6:22
  SPEAKER_01: They are alive, all of their life processes are there, but they're not working because there's no water.
• 6:28
  SPEAKER_02: You know, that is yeah, that is so fascinating because I mean, you know, um if I'm familiar with, for example, uh art artificial insemination on the farm, you know, where you can keep uh uh semen and in liquid oxygen, I think it is, uh, for a long, long time.
• 6:46
  SPEAKER_02: I'm also quite familiar that there are there are seeds.
• 6:51
  SPEAKER_02: Um, you know, uh clover, for example, red clover seeds will last for a thousand years.
• 6:59
  SPEAKER_02: Um so so you know, longevity in dormant longevity is in lots of places.
• 7:08
  SPEAKER_02: What you're telling me though is microbes can do dormant longevity almost beyond the imagination.
• 7:16
  SPEAKER_02: Is that what you're saying?
• 7:18
  SPEAKER_01: Essentially, you know, you also if once you look at the genetic material of a bacterium versus the genetic material uh material of a seed, I mean the seeds are orders of magnitude much more complex.
• 7:33
  SPEAKER_01: So it becomes more of a challenge for the for that seed to maintain the genetic material in a functional form as compared to a bacterium who may have six one or two million base pairs.
• 7:48
  SPEAKER_02: Uh-huh.
• 7:48
  SPEAKER_02: So the simpler, the simpler the life form, the the longer it can, the longer it can stay dormant and and yet be um yet be viable.
• 7:59
  SPEAKER_02: So can you just can you just give us a couple of the little, I don't know, protocols in in layman's terms somehow, um of how you how you do CPR on a on a 10,000-year-old dormant microbe at the bottom of a of a gas drilling well.
• 8:22
  SPEAKER_01: Okay, well, I can give you what I do uh or what I used to do.
• 8:28
  SPEAKER_01: Um you know, first of all, the the first thing that needs to be done is to ensure that the sample you're collecting is actually free of contamination.
• 8:40
  SPEAKER_01: Because it's really, you know, if you have an organism that is dormant and it's can and a sample is contaminated with a viable organism from nature, you know, from current or uh you know actual life, then of course this one, the contaminant will grow faster.
• 8:56
  SPEAKER_01: So first you have to ensure uh almost uh to a great extent that you're not contaminating the product, the sample.
• 9:08
  SPEAKER_01: Second, uh I go on the the assumption that if you have been starving to death in the in the Sahara Desert and you fed Ferrucini Alfredo the first meal, you're going to have uh you're gonna be pretty sick.
• 9:29
  SPEAKER_01: So, what we do is develop uh a protocol in which we use culture media that are that has all of the necessary elements and we dilute them so that we feed them incrementally more concentrated nutrients in a culture medium that will be suitable for their growth.
• 9:50
  SPEAKER_02: And once that is done, yeah, you you you feed them some, you just feed them some chicken broth.
• 9:57
  SPEAKER_01: Exactly, and and with a little bit of salt, not too much fat, uh, and basically that's it.
• 10:04
  SPEAKER_01: Yeah, okay, all right.
• 10:05
  SPEAKER_01: That that's what's next.
• 10:08
  SPEAKER_01: Well, and then we uh once we have ensured that his organism is growing, then what we do is try to ascertain that it's a pure culture using standard microbiological methods.
• 10:25
  SPEAKER_01: Um and that's once you have pure cultures, you have individual colonies that are basically clones of a single organism.
• 10:36
  SPEAKER_01: Then we unfortunately for them, we put them either in liquid nitrogen or we freeze them at minus 80 degrees so they go from being dormant to enjoying life for a few minutes or a few days and then back into the freezer until we have the time to take them out and start looking at them.
• 11:00
  SPEAKER_01: And um, and this is what we have been doing for the last three or four years.
• 11:09
  SPEAKER_01: You know, we isolated most of these organisms in the late 90s, and they were isolated, verified for purity, and then frozen because we were in the process of generating large numbers of different organisms, and then now we had going back to the what we call the culture collection and taking them one at a time and restoring them and analyzing them, both the physiological characteristics that they have as well as the genetic properties, and then we have like a database of organisms and their functions, and that's what we do.
• 11:57
  SPEAKER_00: And you've actually created a product already, right?
• 12:00
  SPEAKER_00: I think one is Paleogrow, and you've tested this on.
• 12:05
  SPEAKER_00: Can you talk about that a little bit?
• 12:07
  SPEAKER_00: What did you test and what results did you see?
• 12:10
  SPEAKER_01: Yeah, what we did was looking at this large collection of bacteria, we looked at the genomes and identified five or six bacteria that would work synergistically for a specific process.
• 12:28
  SPEAKER_01: One such process would be the production of growth promoting substances so that we can accelerate uh seed germination as well as uh plant growth and the ability of the plant to take into the cells uh nutrients from the soil.
• 12:50
  SPEAKER_01: And that we call paleo growth.
• 12:52
  SPEAKER_01: And we also developed another similar product, but the bacteria that we selected were primarily analyzed for the ability to break down uh organophosphates, specifically glyphosate.
• 13:11
  SPEAKER_01: Um, and they are both similar but not identical consortia of bacteria that were genetically evaluated so that they can not only break down the product but be sustainable as a community in the soil.
• 13:30
  SPEAKER_00: Okay, let's start with the products that you created to break down glyphosate, right?
• 13:35
  SPEAKER_00: Because our audience in particular knows a lot about glyphosate at this point, right?
• 13:40
  SPEAKER_00: And we know how ubiquitous it is, and we spend a lot of time trying to avoid glyphosate and also get it out of our bodies.
• 13:47
  SPEAKER_00: And so I find it fascinating that you have found a way to degrade it, not just to AMPA, right?
• 13:54
  SPEAKER_00: The breakdown product, that you have found a way to break it all the way down to CO2, water, and phosphate.
• 14:00
  SPEAKER_00: So what are your, and I knew I know you did some um initial testing.
• 14:05
  SPEAKER_00: Um, what were the results of that testing?
• 14:07
  SPEAKER_00: Like how how much of the glyphosate is actually broken down to CO2, water, and phosphate?
• 14:14
  SPEAKER_01: Well, uh, well, if given sufficient time, and I'll say sufficient time, I'm not measuring in terms of years, but in terms of months, uh, you can remove most of the detectable glyphosate uh with exposure to sufficient concentrations of this consortium of paleo restore.
• 14:40
  SPEAKER_01: And uh so the the in our study uh we predicted that over the six-month period, most of the measurable glyphosate would not be present.
• 14:52
  SPEAKER_01: It will be degraded by the microbial consortium.
• 14:56
  SPEAKER_00: And that's what you actually saw was most of it, the majority of it was degraded over the six months.
• 15:03
  SPEAKER_01: Yes, yes, and uh we started, you know, with a relatively high concentration.
• 15:10
  SPEAKER_01: We started with about 5,000 parts per billion.
• 15:13
  SPEAKER_01: So, and and that's usually you know, probably three lugs of magnitude greater than you find in soil.
• 15:22
  SPEAKER_00: Wow, well, that's remarkable.
• 15:24
  SPEAKER_00: So I know if I'm listening to the show right now, my first thought is where can I get that?
• 15:30
  SPEAKER_00: Right?
• 15:30
  SPEAKER_00: I want some of that product.
• 15:32
  SPEAKER_00: Is it is it is it available for commercial um purchase?
• 15:37
  SPEAKER_01: Yeah, actually, we are currently doing a large scale production, and I'm talking about 10,000 liters production of Paleo Restore.
• 15:49
  SPEAKER_01: And we are packaging them either for individual use in terms of uh eight-ounce containers or for industrial applications in terms of five-gallon jerry cans.
• 16:04
  SPEAKER_01: Uh, so the product is commercially available.
• 16:08
  SPEAKER_01: Uh we we have not we have established the product process for developing, but we have not yet uh put it on a website, which is going to appear within the next three or four weeks.
• 16:24
  SPEAKER_01: So uh we're at we're it's not available commercially in any, and we would like to find someone who's willing to distribute our product, but right now it's basically in our warehouses in jerrycans.
• 16:40
  SPEAKER_00: Wow.
• 16:41
  SPEAKER_00: You know, this got me to thinking, um, and Joel, I'd love you to chime in.
• 16:45
  SPEAKER_00: So when I found out about Paleo Restore, I thought, wouldn't this be a fantastic product for let's say somebody who was trying to convert land that was used to grow crops conventionally, that they're trying to convert that land into um like organic or regenerative farmland?
• 17:03
  SPEAKER_00: I mean, don't you think, Joel, wouldn't that be a great asset to have?
• 17:07
  SPEAKER_02: Yeah, I would think so.
• 17:08
  SPEAKER_02: I mean, I I would ask, I would ask Dr.
• 17:11
  SPEAKER_02: Canno, what what what are you, what do you foresee?
• 17:15
  SPEAKER_02: I mean, you're really in this space.
• 17:16
  SPEAKER_02: What do you foresee as the as the whatever, the the normal client or the normal, the normal customer?
• 17:24
  SPEAKER_02: Is it a gardener uh who's just bought a little homestead and really wants to make sure their garden is clean, or is it or is it uh a more commercial you know, farmer wanting to clean up a soybean field?
• 17:37
  SPEAKER_02: Yeah, well, the answer is all of the above.
• 17:40
  SPEAKER_01: Uh I have uh you know, we have uh a study uh under underway in California in which we are looking at basically a home farmer has one acre of land that's been contaminated with glyphosate, and we provided uh the farmer with uh a free sample so that he can actually treat the soil and of course uh with a caveat that he would need to share the results.
• 18:09
  SPEAKER_01: And we also are working with a um an organic farmer, I want to say in Wisconsin, uh a farmer in Wisconsin who would like to get his land ready for organic uh regenerative farming.
• 18:25
  SPEAKER_01: And uh so we provided him also with a free sample uh of uh paleo restore in order for him to uh test it.
• 18:39
  SPEAKER_01: And uh we are you know, based on what we've looked at in our laboratories and field studies, I'm very hopeful that it will be a successful uh endeavor.
• 18:50
  SPEAKER_01: And therefore, between uh our better manufacturing processes as well as the more supportive data that we're generating, uh that in fact this would be primarily a tool to restore soils for organic farming or even restore soils for better yields.
• 19:15
  SPEAKER_01: Sure.
• 19:15
  SPEAKER_02: So so I I know I know that um a lot of soil can be or um toxicity in soil can be ameliorated simply by bringing up the organic matter, you know, getting the getting the humus up and and you know, with composting and getting a lot of um organic matter up.
• 19:37
  SPEAKER_02: I I call organic matter the the elka seltzer of the soil, you know, it it it it it's uh it's a it's a buffering agent.
• 19:45
  SPEAKER_02: Um speak to the uh speak to the whatever the difference between, for example, uh buffering it out with um heavy applications of compost versus you know using using uh paleo restore.
• 20:02
  SPEAKER_01: Well, um the composting process, uh, you know, actually what it does is increases the organic matter.
• 20:11
  SPEAKER_01: And I presume that in parallel with this uh infusion of um organic material, you're infusing it with water.
• 20:20
  SPEAKER_01: And therefore, what you're increasing is the microbial diversity of the soil.
• 20:25
  SPEAKER_01: And where they're serendipitous or by by intention, they would be microorganisms in the soil that would uh, and there are microorganisms in the soil that will uh break down glyphosate.
• 20:40
  SPEAKER_01: Uh, this is more of a um of a trial and error situation in the case of failure restore.
• 20:49
  SPEAKER_01: Uh, it is a combination of microorganisms that in fact have been shown to break down glyphosate.
• 20:56
  SPEAKER_01: So it's a more sure thing.
• 20:58
  SPEAKER_01: But when we apply it, uh we apply it in an aqueous form, so we're introducing water as well.
• 21:04
  SPEAKER_01: And uh, since the microorganisms are grown in the presence of molasses, we are providing the microorganisms sort of like a nutrient kickstart so that they can become established in the soil and then uh participating not only in restoration, but in also in plant feeding.
• 21:26
  SPEAKER_01: So that's basically the that you know, as I see the difference between the two processes or the two methods.
• 21:34
  SPEAKER_02: So that that's that's fair because because I know that um you know that that high organic matter, if you can, if you can build a lot more organic matter in your soil, it just does a lot of of um what ameliorating work in you know a lot of things.
• 21:54
  SPEAKER_02: And this this is the clever speak of some of these uh chemical companies.
• 21:57
  SPEAKER_02: They say, well, it degrades in the soil, but it Only degrades in soil with high organic matter, you know, and so this is kind of the worst mything and the clever speak.
• 22:06
  SPEAKER_02: Um that the fact is, most chemical fertilized fields are very low in organic matter or certainly active organic matter, um not necessarily legacy organic matter, but currently uh organic matter and and so so they don't break down very well.
• 22:23
  SPEAKER_02: Cena, I know you're you're you're you're dying to ask a question.
• 22:26
  SPEAKER_02: Go ahead, Cena.
• 22:27
  SPEAKER_00: Yeah, okay, so okay, so listening to you two um talk back and forth is really intriguing because I'm thinking, so do microbes, modern microbes that we'll find in the soil here, like say at Joel's farm at a regenerative farm, are there microbes now that can break down glyphosate?
• 22:47
  SPEAKER_00: And if so, then why spend like the money to go back to get the ancient microbes to have them break down the glyphosate?
• 22:55
  SPEAKER_00: Are they more efficient at it?
• 22:57
  SPEAKER_00: Or what what's the difference between using modern our current microbes that we have at our disposal versus the ancient microbes?
• 23:05
  SPEAKER_01: That's a loaded question.
• 23:11
  SPEAKER_00: But uh, I finally I got finally got my daughter off my lap, so now I'm I'm in the conversation again.
• 23:17
  SPEAKER_01: I'm thinking like uh uh uh yeah, well again, go get your daughter back.
• 23:23
  SPEAKER_01: Um they uh now really the situation is the following.
• 23:29
  SPEAKER_01: Uh you're looking at two different sets of organisms, organisms that have been exposed to modern environmental stresses and organisms that have never seen it.
• 23:41
  SPEAKER_01: So you're looking at two different genetic pools.
• 23:44
  SPEAKER_01: Uh and uh presumably uh these organisms are able to survive uh in a broader space, you know, broader spectrum of environmental conditions than they are now, they are being selected for just a specific uh environmental set of stresses.
• 24:05
  SPEAKER_01: So I even though both groups of organisms are capable of breaking down glyphosate, that that's not the issue.
• 24:15
  SPEAKER_01: Uh the issue is to have them break down glyphosate and still survive and thrive in the soil environment.
• 24:25
  SPEAKER_01: And uh so um what I'm saying is that probably there's a greater genetic diversity in the ancient microorganisms than there is in them in the modern counterparts.
• 24:40
  SPEAKER_00: Okay, so so if that's the case, then they are more, they could be, for instance, more likely to survive different that they might experience.
• 24:50
  SPEAKER_00: So so in that sense, yeah, they could be more efficient because they might have a higher survival rate, um, you know, in once once they're in the soil.
• 25:00
  SPEAKER_00: Okay.
• 25:02
  SPEAKER_01: And there is another aspect of it.
• 25:04
  SPEAKER_01: Um, you know, microorganisms in nature function of guilds.
• 25:09
  SPEAKER_01: There's a group of coordinated effort to achieve one specific function.
• 25:14
  SPEAKER_01: Uh, it could be, you know, maybe achieved via cross feeding.
• 25:18
  SPEAKER_01: So the administration to the soil of a single organism that has a specific function, for example, breakdown glyphosate, uh, that organism has a relatively low probability of survival when it's actually going to be introduced into a new ecological niche and had to compete with those organisms that are established.
• 25:43
  SPEAKER_01: So I think even though they might be able to break glyphosate in the laboratory, it is not certain that they would be able to, as individuals, uh, function in the soil.
• 25:56
  SPEAKER_01: Uh, when you know we prepare pelliagrow, we not just have one single organism.
• 26:02
  SPEAKER_01: We have a microbial guild of organisms that are uh cross-feeding and therefore they do not have to compete uh with other organisms, you know, as an individual, but they have actually an ecological niche of their own that once introduced into the soil are less likely to be outcompeted by the organisms that are already present in the soil.
• 26:31
  SPEAKER_00: Okay, but so now you're talking about a different product, right?
• 26:34
  SPEAKER_00: Now we're talking about paleo grow.
• 26:36
  SPEAKER_01: Oh no, paleo restore is the same thing.
• 26:38
  SPEAKER_01: Paleo restore is a microbial guild.
• 26:41
  SPEAKER_01: Yeah, the entire company product line is based on the fact that microorganisms in nature function as gills.
• 26:50
  SPEAKER_01: And therefore, we prepare products in the form of gills that are created based on their genetic composition rather than on their name.
• 27:03
  SPEAKER_01: Because, you know, if we look at a probiotic bacterium Lactobacillus acidophilus, we all think that it is a probiotic organism.
• 27:12
  SPEAKER_01: In fact, there are several, many strains of Lactobacillus acidophilus that have absolutely no probiotic value whatsoever.
• 27:21
  SPEAKER_01: And some of them are extremely good.
• 27:24
  SPEAKER_01: So it's just not just the species, but the strain.
• 27:29
  SPEAKER_01: And that's really very important to emphasize that we actually select our organisms for the guild based on their strain composition.
• 27:40
  SPEAKER_00: Yeah, that's a good distinction.
• 27:42
  SPEAKER_00: I'm I'm glad you brought that up.
• 27:43
  SPEAKER_00: And I also want to just bring up that even though you know people call you the real life Jurassic Park scientists, Alvin, you're talking about isolating these strains, you are not genetically engineering anything, correct?
• 27:59
  SPEAKER_01: Absolutely.
• 27:59
  SPEAKER_01: In fact, the our whole mantra is natural.
• 28:03
  SPEAKER_01: We even grow our organisms in nutrients that are organic, certified organic nutrients, because what we are trying to address is a natural process by natural organisms that have not been intervened in any way by uh the mac by genetic engineering or even genetic manipulation.
• 28:29
  SPEAKER_01: And I will go one step further and say that uh the way that we pre when we isolated this organisms, the first isolation we call generation zero.
• 28:42
  SPEAKER_01: And then when we actually have it purified, we call it generation one.
• 28:47
  SPEAKER_01: That generation one was propagated, created generation two, and those were the ones that be frozen.
• 28:55
  SPEAKER_01: So every time we go to uh make a new product, we go to generation two.
• 29:03
  SPEAKER_01: So it's as close to the original strain as it could possibly be.
• 29:09
  SPEAKER_01: So little or no genetic changes have taken place from the original strain.
• 29:15
  SPEAKER_01: And that's really uh a process that you know is time consuming at cost to the process, but it ensures that that all the products are genetically homogeneous.
• 29:32
  SPEAKER_02: So um in in the soil, so the the the other product, paleogrow, um I I've got I've got a question about why these microbes went into hibernation.
• 29:53
  SPEAKER_02: I mean, if if they were if they were soil based at one time, um you would think on the whole planet that something would have survived in the soil somewhere if they have such survival characteristics.
• 30:10
  SPEAKER_02: Um I I have a follow-up when you when you deal with that, but um deal with that and I'll have a follow-up.
• 30:20
  SPEAKER_01: Well, well, this organism, you know, there is a continuum, okay, of organisms that were in nature, uh, let's say two million years ago, and they they have been multiplying and and generating new generations, you know, developing new generations of bacteria over that period of time.
• 30:43
  SPEAKER_01: But some clone or some group of them got trapped, for example, in amber, and all of a sudden that particular genetic generation got frozen in time.
• 30:56
  SPEAKER_01: It just become became a Kodak moment.
• 31:00
  SPEAKER_01: And the same thing with the some of the soil organisms that get washed into sediments, into the bottom of the ocean, they stopped reproducing, but they didn't lose the viability.
• 31:12
  SPEAKER_01: So they have they they had stopped evolving by either a physical or a chemical process that took place many years ago.
• 31:22
  SPEAKER_01: And some of their counterparts did not stop and then continue growing on the, you know, and now we call them modern organisms, but all these modern organisms have somewhere along the line an ancestor.
• 31:36
  SPEAKER_02: So those that so my follow-up then is that those um those microorganisms that you're finding in, for example, natural gas wells, um, those when they were viable up on the planet's surface, they accounted for much of the the um the ancient flora and fauna.
• 32:04
  SPEAKER_02: I mean, we know, I mean, from from mastodons to dinosaurs to whatever, uh, and and and there had there had to have been a lot of lushness that we can't imagine today.
• 32:16
  SPEAKER_02: Is that right?
• 32:18
  SPEAKER_01: That is basically the were the composition of the ancient environment in which they encountered.
• 32:25
  SPEAKER_01: Well, actually, it is ancient to us now, but to them was contemporary.
• 32:30
  SPEAKER_02: Sure, sure, yeah.
• 32:31
  SPEAKER_02: Uh huh.
• 32:32
  SPEAKER_01: So uh so they represented the microbial diversity, at least a slice of the microbial diversity that existed at that time, were the dinosaurs or the mastodons or whatever, you know, whatever geological era we are looking at.
• 32:52
  SPEAKER_01: Uh so yes, but then if they stopped genetically evolving because of the physical or chemical phenomenon, then they sort of became a representative slice of that time.
• 33:07
  SPEAKER_01: And then those who were not, you know, entrapped in any way, they continue to evolve and they're comprised now, and they're evolving along with the composition of the soil and the environment in which they live.
• 33:23
  SPEAKER_01: So you can have a continuum of genetic diversity or genetic non diversity over time, and but you have this bits and pieces of entrap corte moments in in the evolution of life that are entrapped in some sort of like, for example, amber.
• 33:49
  SPEAKER_02: Well, it's like uh it's like the the mast the mastodons uh as when Siberia, when Siberia thaws, and they're finding these mastodons with uh with grass in their mouths.
• 34:02
  SPEAKER_02: Um obviously that obviously that that wasn't tundra at one time.
• 34:07
  SPEAKER_01: Exactly, exactly.
• 34:09
  SPEAKER_02: Well, we're about out of time.
• 34:11
  SPEAKER_02: You know, on yeah, yeah.
• 34:14
  SPEAKER_02: Um go ahead.
• 34:15
  SPEAKER_02: Fascinating.
• 34:16
  SPEAKER_02: Uh we're about out of time.
• 34:16
  SPEAKER_02: Cena, do you have an another burning uh issue here before we close out?
• 34:21
  SPEAKER_00: I do.
• 34:22
  SPEAKER_00: So I have two follow-up questions.
• 34:24
  SPEAKER_00: Um, because I could just see people in the audience wanting to know this.
• 34:28
  SPEAKER_00: Is there you talked about um the paleo to restore, to break down glyphosate in the soil and the paleo grow?
• 34:36
  SPEAKER_00: Um, right, to help build um uh micronutrients.
• 34:41
  SPEAKER_00: I'm sorry, to help build um microbial diversity in the soil.
• 34:46
  SPEAKER_00: Is there a product for humans, like a human probiotic that you're working on?
• 34:51
  SPEAKER_00: And can the paleo restore be used in water to break down glyphosate that it would be in the water?
• 34:59
  SPEAKER_01: Uh that's uh let me ask answer the second question first.
• 35:04
  SPEAKER_01: I think uh it will require some sort of bioreactor in which the paleo restore is in some sort of column so the water passes through it, and the microorganism will be working on it.
• 35:23
  SPEAKER_01: In fact, we filed a patent uh about two years ago, which is in the in process, uh describing a process in which you could treat water contaminated with glyphosate using some sort of a bioreactor that we have described.
• 35:43
  SPEAKER_01: So the answer to that, yes, right now just in concept.
• 35:50
  SPEAKER_01: So that would require a good engineer to help us out to work this out.
• 35:56
  SPEAKER_01: But I think that's definitely a possibility, and it would actually be, I suspect, even more effective because there's already water, you don't have to water the line.
• 36:08
  SPEAKER_01: Uh and the other question uh a probiotic for humans.
• 36:14
  SPEAKER_01: Um the answer is yes.
• 36:20
  SPEAKER_01: Uh we have not us specifically because we specialize and focus only on environmental microbiology, but the company, the biocollective, has a product.
• 36:37
  SPEAKER_01: I believe they call it sugar shift.
• 36:41
  SPEAKER_01: And in the sugar shift, there is at least one organism that I know of that has the capacity to break down glyphosate through an alternative pathway.
• 36:55
  SPEAKER_01: So, in that respect, uh there is a product that could work.
• 37:02
  SPEAKER_01: I don't know that he has ever been tested.
• 37:06
  SPEAKER_01: Uh the by the bio called paleobiotic are very unlikely we do that because most of our organisms are not what we call generally recognized as safe by the FDA.
• 37:20
  SPEAKER_01: So you cannot consume it.
• 37:22
  SPEAKER_01: So in other words, it's called grass.
• 37:24
  SPEAKER_01: Uh we don't we don't have grass organisms.
• 37:28
  SPEAKER_01: We have organisms that grow on grass, but it's not grass organisms.
• 37:33
  SPEAKER_01: And but the paleo but but the biocollective not only has this organism but has the wherewithal to create one of those one such product.
• 37:43
  SPEAKER_00: Okay, and my last question, because I know we're trying to wrap up here, is um, you've already come amazingly far in in your work.
• 37:51
  SPEAKER_00: And I think this is remarkable.
• 37:52
  SPEAKER_00: Like when we first talked several weeks ago, I was just bouncing around the house, excited, because I have not heard anybody doing this up to this point, especially with the complete breakdown of glyphosate.
• 38:04
  SPEAKER_00: So how can we help you to make this more successful?
• 38:08
  SPEAKER_00: What at this point are you good to go?
• 38:11
  SPEAKER_00: You have everything you need, you can get this out to consumers, or where are you?
• 38:16
  SPEAKER_01: Well, you know, there are several things, you know.
• 38:20
  SPEAKER_01: First of all, money talks.
• 38:22
  SPEAKER_01: And of course, you know, the this lost production batch, uh, you know, it basically is uh a bet uh on a roulette wheel that this is going to be useful and everybody's gonna want it.
• 38:38
  SPEAKER_01: So that's an investment that we already made, but I think the most important thing, as far as I'm concerned, is to have a market, individuals who are interested in purchasing the product for us and uh and and help us distribute it.
• 38:57
  SPEAKER_01: So, in other words, the marketing and sales aspect of the company.
• 39:01
  SPEAKER_01: Right now we are focusing on the production, and I think we are already past uh proof of concept.
• 39:09
  SPEAKER_01: So we we took a chance and manufactured 10,000 liters, which is a lot of acres.
• 39:17
  SPEAKER_01: And we have now a contract with the manufacturer to produce it as we want to and do some product development.
• 39:26
  SPEAKER_01: But the idea is to sell the product, and if we have a partner who can help us do that, even better.
• 39:33
  SPEAKER_00: So you need a partner to help you.
• 39:35
  SPEAKER_01: Yeah, basically, with skills and marketing some sales and distribution.
• 39:40
  SPEAKER_01: That's basically okay.
• 39:42
  SPEAKER_02: So if anybody that that's that's every inventor's nemesis, isn't it?
• 39:47
  SPEAKER_02: Uh you have these they have these genius inventors, but then uh then then finally you need a zigziggler, you know, a zigziggler to uh uh to go to go put it in the in the marketplace.
• 40:00
  SPEAKER_01: Exactly, you know, there and there is a lot of competition, and the only thing that we have going for us is that you know, we are all natural.
• 40:08
  SPEAKER_01: Uh, and probably the price point is gonna be lower than current chemical fertilizers right now by a large margin.
• 40:18
  SPEAKER_01: So, but you know, we don't have this the wherewithal for large-scale distribution and sale.
• 40:27
  SPEAKER_01: That's basically it.
• 40:28
  SPEAKER_01: Right.
• 40:30
  SPEAKER_00: So if somebody is listening and they're interested in partnering with you, how could they get a hold of you?
• 40:35
  SPEAKER_00: Do you want us to put your contact info in the description box for them to reach you, or how would you like that to be handled?
• 40:43
  SPEAKER_01: That would be perfect.
• 40:44
  SPEAKER_01: Uh the cut contact information in the in your uh my personal information.
• 40:50
  SPEAKER_01: Yeah, that would be wonderful.
• 40:51
  SPEAKER_02: Okay.
• 40:52
  SPEAKER_02: We have we have we have numerous, numerous multimillionaire listeners.
• 40:58
  SPEAKER_01: Not me, one of them.
• 41:03
  SPEAKER_02: Uh well, Cena, let's wrap up.
• 41:05
  SPEAKER_02: Uh, this has just been fascinating, and um, I'll let you, I'll let you wrap it up for us.
• 41:11
  SPEAKER_00: Yeah, I think this is just remarkable what you do.
• 41:14
  SPEAKER_00: This could really um propel us forward, right, in this movement of trying to have um a clean um ecosystem, you know, clean food, healthier bodies.
• 41:25
  SPEAKER_00: I mean, this is a solution that I'm actually very excited about.
• 41:29
  SPEAKER_00: And I think a lot of people listening are excited about this.
• 41:32
  SPEAKER_00: I would if anybody's listening and wants to partner with Dr.
• 41:36
  SPEAKER_00: Cano, I mean, he he's a good vet, right?
• 41:39
  SPEAKER_00: I mean, we're in the presence of greatness here.
• 41:41
  SPEAKER_00: And I, and Joel, you know I know a lot of scientists, and you've probably never heard me say that before.
• 41:46
  SPEAKER_00: But yes, he truly is an inspiration and just brilliant.
• 41:51
  SPEAKER_00: So we'll put the contact information in the description box.
• 41:54
  SPEAKER_00: You can get a hold of them.
• 41:55
  SPEAKER_00: And then Dr.
• 41:55
  SPEAKER_00: Cano, when this is available for like for everyday people like me to purchase, will you let me know and come back on the show and tell us how we can get it?
• 42:08
  SPEAKER_01: Yes, I will.
• 42:09
  SPEAKER_01: In fact, we have um through there is a website, and I'll share that with you, Biotiquest website, that we have uh something called Yield and Shield.
• 42:22
  SPEAKER_01: It's a product that is a combination of failure grow and failure restore that we prepare specifically for the biocollective so that they can sell it through the website as Yield and Shield.
• 42:35
  SPEAKER_01: And it comes in eight-ounce containers.
• 42:38
  SPEAKER_01: And I will send you.
• 42:39
  SPEAKER_01: Yeah.
• 42:40
  SPEAKER_00: Okay, great.
• 42:41
  SPEAKER_00: So we'll post that too in the description box.
• 42:43
  SPEAKER_00: So if anyone's interested, they can try it out.
• 42:45
  SPEAKER_00: And then if you do, let me and Joel know what you think about it, right?
• 42:50
  SPEAKER_00: Email me at um handsoffmyfood at 1791.com.
• 42:54
  SPEAKER_00: Dr.
• 42:54
  SPEAKER_00: Kano, this has been so inspiring and enlightening.
• 42:58
  SPEAKER_00: Thank you so much for coming, and we can't wait to have you back on the show again.