731-SLC25A35: for Glycerolipid Synthesis

Paper Talk · Apr 2, 2026 · 25m

This research identifies SLC25A35 as the essential mitochondrial carrier protein responsible for exporting phosphoenolpyruvate (PEP) into the cytosol of lipogenic cells. While PEP is a high-energy metabolite traditionally associated with glucose production, these sources demonstrate that adipocytes primarily generate it within the mitochondria to drive the synthesis of glycerolipids. The study utilizes 13C-pyruvate tracing and liposome reconstitution to prove that SLC25A35 transports PEP in a process gated by mitochondrial membrane potential. Depleting this protein in fat cells impairs…