382-3D Spatial in Thick Tissue Blocks
This research introduces Deep-STARmap and Deep-RIBOmap, advanced imaging platforms designed for 3D spatial transcriptomics and translatomics within thick tissue samples. Traditional methods often struggle with cellular fragmentation and physical distortion during thin sectioning, but these new techniques utilize hydrogel-based re-embedding and covalent crosslinking to preserve genetic information in larger volumes. The authors demonstrate the versatility of these tools by mapping complex neuronal morphologies in the mouse brain and analyzing the tumor microenvironment of human skin cancer. By…
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