1104-Spatial CRISPR Screening with SPAC-seq
The paper introduce SPAC-seq, a high-throughput technology that combines CRISPR gene editing with spatial transcriptomics to map functional genomics within complex tissue environments. Accompanied by a statistical toolkit called TARDIS, this framework allows researchers to link specific gene perturbations to their physical location and biological impact in vivo. The study utilizes these tools to uncover how the loss of Icam1 facilitates tumor metastasis by creating immunosuppressive niches and how CD44 acts as a critical regulator of T cell infiltration and survival. By disrupting the…
The skinny
The skinny isn't ready yet — notes appear once the transcript is processed.