1226-VIPerturb-seq: Genome-Wide Single-Cell CRISPR Screening
The paper introduce VIPerturb-seq, a versatile platform designed to make genome-wide single-cell CRISPR screens more efficient and affordable. By utilizing a synthetic split-barcode system and probe-based detection, the method bypasses the high costs and technical hurdles of traditional sequencing workflows. This approach supports fixed-cell profiling, allowing researchers to specifically isolate and sequence cells exhibiting target phenotypes, such as high protein levels, prior to analysis. Additionally, the platform integrates combinatorial indexing to enhance experimental throughput by up…
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