290-Perturb-Seq: Single-Cell Profiling of Genetic Screens

Paper Talk · Dec 3, 2025 · 21m

The paper introduces and explains Perturb-seq, a new experimental and computational technology that combines pooled CRISPR-based genetic screening with single-cell RNA sequencing (scRNA-seq) to analyze complex biological circuits. This method allows researchers to study the transcriptional effects and genetic interactions of numerous perturbations simultaneously within individual cells. The accompanying computational framework, MIMOSCA, is designed to estimate the impact of these perturbations on gene expression, cellular states, and gene programs, proving robust even when accounting for…