1700-OpenFISH Same-Section Spatial Transcriptomics

Paper Talk · Oct 23, 2026 · 26m

Researchers have developed OpenFISH, an open-source and cost-effective spatial transcriptomics platform operating at single-cell resolution to map gene expression within tissues. A primary breakthrough of this technology is its ability to integrate same-section spatial transcriptomics with matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), overcoming previous hardware and chemical incompatibilities. By utilizing poly-L-lysine-coated indium tin oxide slides and optimized tissue-clearing protocols, the workflow eliminates laser-induced autofluorescence while…