221-RNA-Stabilized Coat Proteins for Single mRNA Imaging
The paper introduce novel RNA-stabilized coat proteins, named dMCP and dPCP, engineered to overcome the challenge of high background noise in live-cell RNA imaging. These variants of the traditional MS2 and PP7 coat proteins are designed to rapidly degrade in the absence of their target RNA ligands, ensuring that only the RNA-bound proteins produce a fluorescent signal. Through techniques like circular permutation and degron masking, the researchers achieved up to a 63-fold increase in the stability of dMCP when bound to its cognate MS2 RNA. The new probes allow for sensitive, low-background…
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