252-Multiscale Integrated Cell Map of Protein Assemblies

Paper Talk · Nov 21, 2025 · 24m

The paper outlines the creation of a global, multiscale map of human subcellular architecture in U2OS osteosarcoma cells, integrating two complementary data sources: biophysical protein interactions determined by affinity purification–mass spectrometry (AP–MS) and protein localization images from immunofluorescence (IF). The researchers employed a self-supervised machine learning model to fuse these modalities, resolving 275 distinct molecular assemblies spanning a broad range of physical sizes, which they systematically validated using whole-cell size-exclusion chromatography (SEC–MS). This…